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Briefly, a total reaction volume of 10 µl was prepared by combining 1 µl of cDNA, 5 µl of SYBR green (2x) mixes, and 0.5 µl each of forward and reverse primers

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Epigenetic changes, telomere shortening and DNA damage, proteotoxicity, mitochondrial dysfunction, and changes in intercellular communication and key signaling pathways (MAPK, 176 JAK-STAT3, 177 and Notch 178 ) all lead to permanent cell cycle arrest and exhaustion of satellite cell pools