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For further experimentation, BMMs were cultured with M-CSF (30 ng/mL) and receptor activator of nuclear factor kappa-B ligand (RANKL, 50 ng/mL)

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In the current study, measurement of plasma dUrd concentration showed that pemetrexed administered at doses of 400–700 mg m −2 produced consistent elevations in plasma dUrd, suggesting that plasma exposure to pemetrexed was sufficient to induce TS inhibition in patients