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With the assumption of a steady-state laminar flow profile in the device, we calculated the maximum shear stress at the bottom of the cell culture chamber to be approximately 0.0025–0.013 dynes/cm 2 according to the following equation (1): where µ = flow viscosity (kg/m*s), Q = volumetric flow rate (m 3 /s), h = channel height (m), and w = channel width (m) 35