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Under satisfactory anaesthesia, abdominal cavity was insufflated using a 1.5 l min −1 insufflator and Veress needle until the abdomen is distended and tympanic on percussion

gold

Age group:Teens

The liver tissue was homogenized with a buffer solution having 250 mM sucrose, 50 mM Tris-HCl (pH 7.4), and 1 mM EDTA and stored with 4% paraformaldehyde (PFA) or at –80°C

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