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Intranasal administration of peptides has been shown effective due to the following factors [27]: Large surface area Permeable endothelial membrane Elimination of first-pass effect Good circulation Researchers who wish to administer the BPC-157 and TB-500 blend via a nasal spray are encouraged to take certain precautions to ensure delivery of maximum bioavailability: To optimize absorption: Wait up to one minute between sprays via the same nostril to prevent oversaturating the nasal membrane and thus losing peptide due to swallowing

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Van Itallie et al 44 analyzed flux of polyethylene glycols across pig ileum and monolayers of Caco-2 intestinal epithelial cells and 2 distinct clones of Madin-Darby canine kidney (MDCK) cells