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Cell culture Mouse C2C12 myoblasts, rat L6 myoblasts and 3T3-L1 pre-adipocytes were maintained in DMEM supplemented with 10% heat-inactivated FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified atmosphere of 5% CO 2