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Previous in vivo human data have shown that the plasma concentrations of APR-246 in the 12 h following its intravenous administration range from 50 to 500 mM, suggesting that concentrations above 50 mM are suitable for in vitro studies of the early effects of APR-246

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The first hypothesis was proposed by Gilliam and Cox,5 who reported that patients with vitamin B12 deficiency had decreased levels of reduced glutathione (GSH)