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We performed conformational variability analysis (3DVA) 94 in cryoSPARC for the full-length, ATD, and LBD-TMD of all the conformations of GluK2/K5em-5-IW-Kyn and GluK2/K5em-AMPA, and LBD-TMD of previously reported GluK2/K5em-CNQX dataset 23 with a mask that excluded solvent and micelle, with 3 variability components and a low-pass filter resolution of 5.5 Å

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Rats were assessed for fine motoric impairment with modified Irvine, Beatties and Bresnahan forelimb scale (IBB scale) that can detect recovery of both proximal and distal forelimb function including digit movements during a naturally occurring behavior that does not require extensive training or deprivation to enhance motivation (64)