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Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway

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Isolated hippocampi were digested with 20 U/ml of papain (Worthington) and 2000 U/ml DNase1 (Worthington) at 37 °C for 60 min before being triturated with a 10 ml pipette