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Immunohistochemistry Immunohistochemistry was performed using a Ventana discovery XT for NANOG and Sca1/Ly6A/E, the Leica BOND RX Research Advanced Staining System for H3K36me3, keratin 14 and vitamin B 12 , and manually for Ki67

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This in turn leads to the increased oxidation of fatty acids in other tissues such as muscle and liver