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For analysis of flavonoids by HPLC–MS, reaction volumes were split after the plate reader incubations, and a 40 μl aliquot was extracted with 80 μl of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid for quantification of cyanidin ( 8 ) concentration while a second 40 μL aliquot was extracted with 80 μl of 75% acetonitrile in H 2 O, 1 mM EDTA for quantification of all other flavonoids