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2) prompted us to conclude that GSH1 specific activity did not depend on an over expression of this protein and that other mechanisms may be involved in such a phenomenon

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All experiments were conducted in a buffer solution containing 20 mM HEPES (pH 7.0), 150 mM NaCl, 1 mM dithiothreitol (DTT), and 5% dimethyl sulfoxide (DMSO)