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Medium was changed daily (typically with THR-123 for 5 days and without it for the following 5 days) and slices imaged also daily at 4x (to capture most of the slice) and 20× in specific regions of interest (ROI) that were arbitrarily selected on the first day of treatment using topographical landmarks present in the slice to ensure accurate longitudinal imaging

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[DOI] [PubMed] [Google Scholar] 14.Hsieh M.J., Liu H.T., Wang C.N., Huang H.Y., Lin Y., Ko Y.S., Wang J.S., Chang V.H.S., Pang J.H.S