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After centrifugation at 13,000 × g for 10 min, the supernatant was mixed with an equal volume of 2× SDS sample buffer (125 mM Tris-HCl, pH 6.8, 4% SDS, 30% glycerol, and 10% 2-mercaptoethanol) and then boiled at 95°C for 5 min

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Iron chelators delivered using nanoparticles have been observed to cross the blood–brain barrier (BBB) to deliver the drug to the brain while also effectively preventing metal-associated oxidative damage