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Primary antibodies used were mouse monoclonal anti-Gpx3 (Abcam, Cambridge, UK, 1:50 or 1:100) and rabbit polyclonal anti-Collagen I antibody (Rockland, Gilbertsville, PA, USA, 1:200)

lab-supply

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RNS include nitric oxide radical (NO or NO ● ), nitrite (NO 2 − ), nitrogen dioxide radical (NO 2 ● ), and peroxynitrite (ONOO − ) [20]