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Subsequently, all unique sequences were aligned against the SILVA v138.1 reference alignment from the mothur repository ( Taxonomic classification was assigned to amplicon sequence variants (ASVs), retaining only those attributed to archaeal and bacterial domains

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Determination of Michaelis-Menten constant for monobromobimane For validation of the assay, the enzymatic reaction between monobromobimane and glutathione was characterised to determine kinetic parameters such as apparent Michaelis-Menten constant and maximum rate of the reaction