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This work provides essential details for the advancement of 197m/g Hg 2+ radiopharmaceuticals, demonstrating the need for the development of specific and custom chelators for these exotic soft radiometals, as the use of these commercial chelators is not feasible for future advancement of the theranostic pair 197m/g Hg 2+ in nuclear medicine applications due to the TCMC-PSMA bioconjugate’s poor in vitro stability against HS and GSH, suboptimal apparent molar activity and deficient K i values