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Then, we carried out an in vitro methylation assay of the 114-mer RNA segment with either the wild-type TRMT61B or the D335A mutant in the presence or absence of AdoMet, followed by RNase A digestion, and subjected them to capillary LC/nano-ESI-MS analysis

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Using a multi-omics analysis and genetic manipulations, another study identified early 2 factor transcription factor 4 (E2F4), transcriptional enhanced associate domain transcription factor 1 (TEAD1) and activating protein-1 (AP-1) to be key factors regulating the RS transcriptome and epigenome