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The cells were lysed on ice for 30 min in 100 μl lysis buffer, (120 mM NaCl, 40 mM Tris pH 8.0, and 0.1% NP 40) prior to being centrifuged at 13,000 × g for 15 min

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doi: 10.1007/s11356-016-7295-8 [DOI] [PubMed] [Google Scholar] Najami N., Janda T., Barriah W., Kayam G., Tal M., Guy M., et al