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Research on ischemic tissues shows blood vessel formation increases by 129 to 152 percent compared to untreated controls
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To ensure sufficient and undisturbed tissue sampling for different analyses, a bilateral collection strategy was used: the right gastrocnemius muscle was fixed in 10% neutral-buffered formalin and reserved for histopathological and immunohistochemical evaluations, while the left gastrocnemius muscle was used for quantitative real-time polymerase chain reaction (qRT-PCR) analyses