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When staining was performed with anti-GCLC, anti-cleaved caspase3, anti-TSPO (PBR), anti-PSD95 or anti-GSDME antibodies, we applied a fluorescence-indirect tyramide signal amplification (TSA) technique (TSA System

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For the abdomen, stay at least two inches away from the navel and avoid any areas with scars, bruises, or stretch marks

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