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The supernatant was then incubated with click reaction components [Tris-(benzyltriazolylmethyl) amine (100 μM), CuSO 4 (1 mM), Tris-carboxyethylphosphine (1 mM), and azido-biotin (100 μM)] at 37°C for 30 min, protected from light
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This bright yellow compound has a long history of use in traditional Chinese and Ayurvedic medicine