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Isopropyl-β-d-thiogalactopyranoside (IPTG) was added into the culture to a final concentration of 0.1 mM for GST-fusion protein or 0.3 mM for MBP-fusion protein when the culture reached an absorbance of ~0.4 at 600 nm

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[23] demonstrated that switching from red meat consumption to white meat or non-meat protein reduced plasma and urine TMAO over one month

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