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Studies showed that TGF-β induces the expression of the de novo serine [phosphoglycerate dehydrogenase (PHGDH), phosphoserine aminotransferase 1 (PSAT1) and phosphoserine phosphatase (PSPH)] and glycine [serine hydroxymethyltransferase 2 (SHMT2)] synthesis pathways in human fibroblasts (225)

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A ChemiDoc TM XRS imaging system (Bio-Rad, United States) was used to visualize the signals