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Chondrocytes proliferation and apoptosis assay The mutant or control chondrocytes were plated in six-well plates with the density of 1×10 5 , and cultured in medium with 1% serum for 24 h and then were restimulated with insulin (10 nmol·L −1 ) for an additional 24 h before harvest

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Sermorelin therapy can be used short or long-term depending on your goals