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2.4 Biochemical assays Plasma insulin, GLP-1, glucagon, and lipopolysaccharides (LPS) were measured using the Ultra Sensitive Mouse Insulin ELISA Kit (Crystal Chem, 90080), the V-PLEX Plus GLP-1 (active) Kit (mesoscale, K1503OG), the Mouse Glucagon ELISA Kit (Crystal Chem, 81518), and the Lipopolysaccharides (LPS) ELISA Kit (abbexa, abx150357) following the manufacturer’s instructions

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