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After 4–8 h, DMEM was replaced with Neurobasal medium (Gibco, 21103049) containing 50 × B27 (Gibco, 17504044), 100× glutamine (Invitrogen, 25030081), and 100× penicillin-streptomycin (Thermo, 15140122)

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Metabolic studies of a synthetic lipolytic domain (AOD9604) of human growth hormone in vitro and in vivo