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Detailed kinetic analysis, enzyme inhibition and X-ray crystallography studies of piperazines 156 (IC 50 = 0.12 μM) and its synthetic analogue 157 (IC 50 = 0.080 μM) [229], disclosed their mechanistic behaviour as substrate-selective, slow-binding inhibitors of gut bacterial βGLU with similar pharmacological profile as compound 151

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Role of glutathione in the export of compounds from cells by the multidrug-resistance-associated protein