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Replacement of Tyr110 in recombinant Y110A decreased both k cat app and 1/Φ values for the disulfide substrate and GSH in the GSSCys and HEDS assay (Supplementary Figs 18 and 19)

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Work backwards: you need concentration = dose ÷ draw volume = 0.5 mg ÷ 0.25 mL = 2 mg/mL