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For colocalization immunofluorescence staining, the slides were incubated with Alexa Fluor conjugated secondary antibody (Invitrogen, #Alexa Fluor 568-A11011, Alexa Fluor 488- A11029) for 2 h at room temperature

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The peptide’s mechanism of triggering gene expression changes that persist independently may make precise timing less critical than with compounds that require constant presence at receptors

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