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WT FSP1 in Cas9 control and FLAD1 KO cells was highly stable with a half-life of approximately 24 h, whereas WT FSP1 in RFK KO cells exhibited significantly reduced stability with a half-life of approximately 6 h (Fig

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Using the same method, we generated epithelioid cultures of human esophageal epithelium from transplant donors aged 36–76 years (Fig