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Chromatin was cross-linked with 1% formaldehyde at room temperature for 10 min and sonicated in SDS-lysis buffer (1% SDS, 50 mM Tris-HCl pH 8.0, 10 mM EDTA pH 8.0, with protease inhibitor) using a Bioruptor (Cosmo Bio, Tokyo, Japan)

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[DOI] [PubMed] [Google Scholar] 2.de Sousa T.R., Dt C., Novais F