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This raises the need for maintaining a closely similar method of preparations for Aβ experiments, as the method of peptide preparation, peptide concentration, time and aggregation, and model system used may play a huge role in determining the role of DiY on Aβ toxicity ( On the other hand, DiY cross-linking on existing fibrils might stabilize and help to maintain insoluble Aβ species found in plaques in the AD brain (Wang et al., 1999