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Excess antibody was washed three times with blocking solution and the cells were incubated with secondary antibody (Cy3 anti mouse, diluted 1:500, Jackson cat: 715-165-151) for 1 h at room temperature

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Specification:50 mg vial

A 1 mL U-100 insulin syringe with a fixed integrated needle in the 28G to 31G range and a 0.5 inch length