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By using an in vitro assay with mitochondrial lysates (i.e., in conditions where the original mitochondrial stoichiometry is lost), we observed a trend to phenocopy such inhibition by adding acetyl-CoA to FASN lox/lox -PyMT lysates: pyruvate dehydrogenase activity was similar to that of FASN ∆/∆ -PyMT MEFS, but not statistically significantly different—albeit lower—to that of FASN lox/lox -PyMT MEFS (Supplementary Fig

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Chemical grade:Laboratory

10.3390/ijms17071166 [DOI] [PMC free article] [PubMed] [Google Scholar] Jones S

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