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Male C57BL/6 mice (7–8 weeks old, 25–28 g) were purchased from the Institute of Laboratory Animal Science at the Chinese Academy of Medical Sciences (Beijing, China) and housed in a specific pathogen-free facility maintained at 20–22 °C, with cycles of 12 h light-dark and food and water ad libitum

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The omega-3 index measures the concentration of EPA and DHA relative to all other fats in red blood cell membranes and is a validated method of assessing tissue omega-3 fatty acid concentrations