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Interestingly, the variants of surface exposed residues belong to two different domains of FXN within the NFS1-ISCU-FXN complex: the region spanning Glu96 to Asp124, corresponding to the first α-helix of FXN that is involved in the interaction with NFS1, and the region spanning Asn146 to Arg165 composing the β-sheet that is involved in the interaction with ISCU (Figure 4D)