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News Release July 31, 2003 ConsumerLab.com finds many cholesterol-lowering supplements poorly made — Test results published online today WHITE PLAINS, NY — July 31, 2003 — ConsumerLab.com reported today that more than half of the cholesterol-lowering supplements that it recently purchased failed to contain their listed ingredients and/or could not adequately break apart to release their contents

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Samples (30 µg protein) were resolved by 4–20% SDS-PAGE (Biofuraw™ Precast Gel, Tanon Science, Shanghai, China), transferred to polyvinylidene difluoride membranes, and incubated with one of the following antibodies: LC3 (88588s, Cell Signaling Technology, 1:1000), GPX4 (ab125066, Abcam, 1:1000), p16 (1003-1-AP, Proteintech, 1:1000)